The long-legged myotis lives in forests and comes out early in the evening to hunt. It is a fast flier and will chase insects for a long distance.
One baby is born in the summer, and the species hibernates in winter.
In general, the long-legged myotis is a species associated with montane coniferous forests, but it also occurs in some desert and riparian habitats.
The long-legged myotis is an Oregon Conservation Strategy Species in these ecoregions: Blue Mountains, Coast Range, East and West Cascades, Klamath Mountains, and Northern Basin and Range.